化学発光免疫測定法(ChLIA)

EUROIMMUN ChLIA

ランダムアクセス・高速処理

EUROIMMUN ChLIA の特長

  • 全自動アッセイ  IDS-iSYS Multi-Discipline Automated System/IDS-i10によりChLIAアッセイの全自動化。システム連携によりLISから装置ステータスや結果確認が可能
  • 高速処理 –  測定から結果まで約25分
  • 専用カートリッジ試薬  キャリブレーション試薬もキットに付属し、装置での冷蔵保存が可能
  • QCデータを自動管理 専用試薬はRFIDタグで管理。試薬管理の労力とヒューマンエラーを低減
  • 定量測定 数週間以上安定なキャリブレーション曲線に基づき定量測定
  • 広い測定ダイナミックレンジ 高感度かつ特異性の高いキャリブレーション試薬により広いダイナミックレンジでの定量測定

  • Test principle

    Chemiluminescence immunoassays (ChLIA) from EUROIMMUN use magnetic particles coated with antibodies or antigens as a solid phase to detect specific antigens or antibodies in patient samples by means of chemiluminescence signals. Performance and evaluation are fully automated with the IDS-iSYS or the IDS-i10.


    Antibody detection by ChLIA

    The antigen-coated magnetic particles are incubated with diluted patient samples. If a sample contains specific antibodies directed against the antigen, these bind to the antigen-coated magnetic particles. In the next step, acridinium-labelled antibody (conjugate) is added. This binds to the specific antibodies.  A trigger solution is then added to induce a chemiluminescence reaction. The resulting light signal is proportional to the antibody concentration in the patient sample within the measurement range and is automatically converted into a concentration by the instrument.


    Antigen detection by ChLIA

    The antibody-coated magnetic particles are incubated with the patient sample and an antigen-specific biotinylated antibody. During the incubation, the antigen is bound by the magnetic particle-coupled antibody as well as by the biotinylated antibody. In the next step, acridinium-labelled ExtrAvidin (conjugate) is added. This binds to the biotinylated antibody. A trigger solution is then added to induce a chemiluminescence reaction. The resulting light signal is proportional to the antigen concentration in the patient sample within the indicated measurement range and is converted automatically into a concentration by the instrument.


  • Automation

    IDS-i10

    Product

    IDS-iSYS

    Product

  • Files

Techniques

蛍光抗体法

Technique

ELISA法

Technique

イムノブロット

Technique

ChLIA法

Technique

マイクロアレイ

Technique

リアルタイムPCR

Technique
[Translate to Japanese:] Dried Blood Spots (DBS)

サンプル収集と事前分析

Technique
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