The Pre-NAT II allows fully automated high-throughput sample preparation for molecular genetic diagnostics, consisting of nucleic acid extraction and subsequent pipetting of the PCRs. In this way, up to 96 primary samples from various source materials are prepared efficiently, safely and without any manual intermediate steps for subsequent PCR-based tests, e.g. EUROArray and EURORealTime.
After automated barcode identification of the sample tubes, the cell material is lysed and the nucleic acids are extracted by means of magnetic particles. These are subsequently transferred one after another into the automatically dispensed reagents by magnetisable metal rods. In this way, there is no risk of cross-contamination during the transport of liquids. Afterwards, the liquid-handling module of the Pre-NAT II automatically pipettes the prompted PCRs using the extracted nucleic acids and the PCR reagents, which are stored refrigerated in the instrument.